CNS Neurosci Ther. 2017 Apr 21. doi: 10.1111/cns.12700. [Epub ahead of print]
A cross-circulated bicephalic model of head transplantation.
Li PW1, Zhao X1, Zhao YL1, Wang BJ1, Song Y1, Shen ZL1, Jiang HJ1, Jin H1, Canavero S2, Ren XP1,3,4.
A successful cephalosomatic anastomosis (“head transplant”) requires, among others, the ability to control long-term immune rejection and avoidance of ischemic events during the head transference phase. We developed a bicephalic model of head transplantation to study these aspects.
METHODS AND RESULTS:
The thoracic aorta and superior vena cava of a donor rat were anastomosed with the carotid artery and extracorporeal veins of a recipient rat by vascular grafts. Before thoracotomy in the donor rat, the axillary artery and vein of the donor were connected to the carotid and the extracranial vein of the third rat through a silicone tube. The silicone tube was passed through a peristaltic pump to ensure donor brain tissue blood supply. There is no ischemia reperfusion injury in donor brain tissue analyzed by electroencephalogram. Postoperative donor has pain reflex and corneal reflex.
Peristaltic pump application can guarantee the blood supply of donor brain tissue per unit time, while the application of temperature change device to the silicone tube can protect the brain tissue hypothermia, postoperative experimental data show that there is no brain tissue ischemia during the whole operation. The application of vascular grafting can also provide the possibility of long-term survival of the model.
© 2017 John Wiley & Sons Ltd.